Hotspot

atpH-atpI

Genome · 79526-80354 · 829 bp

Back to Phlegmariurus

Pi 0.0586

MarkerSeek score 78.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: Genome
length_bp: 829
variable_sites: 46
pi: 0.0586
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	GCAGCAATAACAGAAGCAGC	TTTAGCTGCTCGGAATCCAC	50.0	50.0	58.1	58.0	1442-1474 bp	1.0	84.5
atpH-atpI_p2	CGCAATACCTTCTACGGCTT	TTTAGCTGCTCGGAATCCAC	50.0	50.0	58.1	58.0	1524-1556 bp	1.0	84.4
atpH-atpI_p3	TTCAGCTTCTGGTTGTCTCG	TTTAGCTGCTCGGAATCCAC	50.0	50.0	57.8	58.0	1542-1574 bp	1.0	83.7
atpH-atpI_p4	TAGACCGAGTACCACACCTG	TTTAGCTGCTCGGAATCCAC	55.0	50.0	58.2	58.0	2133-2145 bp	1.0	82.9
atpH-atpI_p5	CGCAATACCTTCTACGGCTT	TTTTGCAACTTTAGCCGCAG	50.0	45.0	58.1	57.9	1028 bp	0.5	58.4