Hotspot

trnG-UCC-trnR-UCU

Genome · 74570-75901 · 1332 bp

Back to Phlegmariurus

Pi 0.0758

MarkerSeek score 84.20

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: Genome
length_bp: 1332
variable_sites: 97
pi: 0.0758
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	ACCCTTAGCCTTCCAAGCTA	CTGTCCTATCCATTAGACGATGG	50.0	47.8	58.0	57.9	1404-1409 bp	1.0	63.7
trnG-UCC-trnR-UCU_p2	ACCCTTAGCCTTCCAAGCTA	TGTCCTATCCATTAGACGATGG	50.0	45.5	58.0	56.9	1403-1408 bp	1.0	63.1
trnG-UCC-trnR-UCU_p3	TTCCAAGCTAATGATGCGGG	CTGTCCTATCCATTAGACGATGG	50.0	47.8	58.3	57.9	1394-1399 bp	1.0	61.4
trnG-UCC-trnR-UCU_p4	TTCCAAGCTAATGATGCGGG	TGTCCTATCCATTAGACGATGG	50.0	45.5	58.3	56.9	1393-1398 bp	1.0	60.9
trnG-UCC-trnR-UCU_p5	GCTAATGATGCGGGTTCGAT	CTGTCCTATCCATTAGACGATGG	50.0	47.8	58.5	57.9	1388-1393 bp	1.0	60.2