Hotspot

rps4-trnS-GGA

Genome · 40832-41229 · 398 bp

Back to Phlegmariurus

Pi 0.0678

MarkerSeek score 78.20

Primer available yes

Label rps4-trnS-GGA

Feature Metadata

label_name: rps4-trnS-GGA
feature_type: igs
region: Genome
length_bp: 398
variable_sites: 27
pi: 0.0678
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnS-GGA_p1	CACTACAGAGTGTGGGATTGG	CTCTTGCTTACCGAGGGTTC	52.4	55.0	58.0	58.0	539-565 bp	1.0	75.6
rps4-trnS-GGA_p2	CACTACAGAGTGTGGGATTGG	GGGTTCGAATCCCTCTCTCT	52.4	55.0	58.0	58.2	525-551 bp	1.0	74.1
rps4-trnS-GGA_p3	ACTACAGAGTGTGGGATTGGT	CTCTTGCTTACCGAGGGTTC	47.6	55.0	58.4	58.0	538-564 bp	1.0	72.8
rps4-trnS-GGA_p4	ACTACAGAGTGTGGGATTGG	CTCTTGCTTACCGAGGGTTC	50.0	55.0	56.5	58.0	538-564 bp	1.0	72.2
rps4-trnS-GGA_p5	CTACAGAGTGTGGGATTGGT	CTCTTGCTTACCGAGGGTTC	50.0	55.0	56.5	58.0	537-563 bp	1.0	72.2