Hotspot

psbB-clpP

Genome · 14335-15388 · 1054 bp

Back to Phlegmariurus

Pi 0.044
MarkerSeek score 82.60
Primer available yes
Label psbB-clpP

Feature Metadata

label_name
psbB-clpP
feature_type
igs
region
Genome
length_bp
1054
variable_sites
41
pi
0.044
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.88
markerseek_score
82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbB-clpP_p1 AACTAACGCGGTATGCATCA TACGTCAATCCAAACGGCAT 45.0 45.0 57.7 57.9 1114-1213 bp 1.0 83.6
psbB-clpP_p2 ACTAACGCGGTATGCATCAA ATACGTCAATCCAAACGGCA 45.0 45.0 57.7 57.9 1114-1213 bp 1.0 83.6
psbB-clpP_p3 ACTAACGCGGTATGCATCAA TACGTCAATCCAAACGGCAT 45.0 45.0 57.7 57.9 1113-1212 bp 1.0 83.6
psbB-clpP_p4 TAACGCGGTATGCATCAAGT ATACGTCAATCCAAACGGCA 45.0 45.0 57.7 57.9 1112-1211 bp 1.0 83.6
psbB-clpP_p5 TAACGCGGTATGCATCAAGT TACGTCAATCCAAACGGCAT 45.0 45.0 57.7 57.9 1111-1210 bp 1.0 83.6