Hotspot

petB

LSC · 78321-79772 · 1452 bp

Back to Phippsiomeles

Pi 0.00484

MarkerSeek score 56.50

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1452
variable_sites: 7
pi: 0.00484
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	TTGGTAGTTTGACCGTGGAA	AGTGTTCCTACCCCAAGTGA	45.0	50.0	57.0	57.9	1545 bp	1.0	71.7
petB_p2	TTTGGTAGTTTGACCGTGGA	AGTGTTCCTACCCCAAGTGA	45.0	50.0	57.0	57.9	1546 bp	1.0	71.7
petB_p3	TGGTAGTTTGACCGTGGAAT	AGTGTTCCTACCCCAAGTGA	45.0	50.0	56.8	57.9	1544 bp	1.0	69.8
petB_p4	TTTGGTAGTTTGACCGTGGAA	AGTGTTCCTACCCCAAGTGA	42.9	50.0	57.7	57.9	1546 bp	1.0	69.5
petB_p5	TTTTGGTAGTTTGACCGTGGA	AGTGTTCCTACCCCAAGTGA	42.9	50.0	57.7	57.9	1547 bp	1.0	69.5