Hotspot

rpl32-trnL

SSC · 117508-118905 · 1398 bp

Back to Phippsiomeles

Pi 0.00866

MarkerSeek score 64.60

Primer available yes

Label rpl32-trnL

Feature Metadata

label_name: rpl32-trnL
feature_type: igs
region: SSC
length_bp: 1398
variable_sites: 12
pi: 0.00866
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_p1	GGGCAGCATTGAAAGCTTTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.8	58.0	1510-1602 bp	1.0	79.8
rpl32-trnL_p2	GGGCAGCATTGAAAGCTTTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.8	58.0	1511-1603 bp	1.0	79.8
rpl32-trnL_p3	TAGGGCAGCATTGAAAGCTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.8	58.0	1512-1604 bp	1.0	79.4
rpl32-trnL_p4	TAGGGCAGCATTGAAAGCTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.8	58.0	1513-1605 bp	1.0	79.4
rpl32-trnL_p5	GGGCAGCATTGAAAGCTTTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.8	57.9	1520-1612 bp	1.0	78.8