Hotspot

rps11-rpl336

LSC · 82868-83033 · 166 bp

Back to Phippsiomeles

Pi 0.0301

MarkerSeek score 61.90

Primer available yes

Label rps11-rpl336

Feature Metadata

label_name: rps11-rpl336
feature_type: igs
region: LSC
length_bp: 166
variable_sites: 5
pi: 0.0301
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl336_p1	ACGTCTATTTCTACGCGAACC	ACCCGAGACATAAACAAAGACA	47.6	40.9	58.2	57.7	240-242 bp	1.0	53.2
rps11-rpl336_p2	CGTCTATTTCTACGCGAACCA	ACCCGAGACATAAACAAAGACA	47.6	40.9	58.2	57.7	239-241 bp	1.0	53.2
rps11-rpl336_p3	CGTCTATTTCTACGCGAACC	ACCCGAGACATAAACAAAGACA	50.0	40.9	56.5	57.7	239-241 bp	1.0	50.7
rps11-rpl336_p4	CCTTGGGGATTCTACGTGTA	ACCCGAGACATAAACAAAGACA	50.0	40.9	56.3	57.7	277-279 bp	1.0	49.4
rps11-rpl336_p5	ACATGAATAACTCCCTTGGGG	ACCCGAGACATAAACAAAGACA	47.6	40.9	57.3	57.7	290-292 bp	1.0	49.2