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psbE-petL

LSC · 67788-69053 · 1266 bp

Back to Phippsiomeles

Pi 0.00948

MarkerSeek score 64.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1266
variable_sites: 12
pi: 0.00948
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCG	50.0	55.0	57.8	58.2	1381-1411 bp	1.0	78.1
psbE-petL_p2	ACGAATAACCAACCCGCAAT	GTCGTATCTTGCTCAGACCG	45.0	55.0	58.2	58.2	1467-1497 bp	1.0	77.9
psbE-petL_p3	TGCTGACGAATAACCAACCC	GTCGTATCTTGCTCAGACCG	50.0	55.0	58.2	58.2	1472-1502 bp	1.0	77.8
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	GTCGTATCTTGCTCAGACCG	55.0	55.0	58.2	58.2	1376-1406 bp	1.0	77.7
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	TCGTATCTTGCTCAGACCGA	50.0	50.0	57.8	58.3	1380-1410 bp	1.0	77.4