Hotspot

petA-psbJ

LSC · 66011-67018 · 1008 bp

Back to Phippsiomeles

Pi 0.00397

MarkerSeek score 61.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1008
variable_sites: 4
pi: 0.00397
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGCTCTTCTTGGCATCTGTT	AGCCGGTATTCTTGTGATCG	45.0	50.0	57.4	57.8	1179-1244 bp	1.0	73.9
petA-psbJ_p2	TTGCTCTTCTTGGCATCTGT	AGCCGGTATTCTTGTGATCG	45.0	50.0	57.4	57.8	1180-1245 bp	1.0	73.9
petA-psbJ_p3	CGTGTCCAAGGTCTTTTGCT	AGCCGGTATTCTTGTGATCG	50.0	50.0	58.7	57.8	1195-1260 bp	1.0	73.0
petA-psbJ_p4	GGTCTTTTGCTCTTCTTGGC	AGCCGGTATTCTTGTGATCG	50.0	50.0	57.3	57.8	1186-1251 bp	1.0	72.8
petA-psbJ_p5	TCCATTACGTGTCCAAGGTC	AGCCGGTATTCTTGTGATCG	50.0	50.0	57.2	57.8	1202-1267 bp	1.0	72.5