Hotspot

rbcL-accD

LSC · 59581-60148 · 568 bp

Back to Phippsiomeles

Pi 0.00887

MarkerSeek score 63.90

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 568
variable_sites: 5
pi: 0.00887
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGTCCTGAACTAGCTGCTG	CGAATCGAACCGCCATTTTT	55.0	45.0	57.7	57.7	719-725 bp	1.0	76.6
rbcL-accD_p2	GAGTCCTGAACTAGCTGCTG	ATCGAATCGAACCGCCATTT	55.0	45.0	57.7	58.3	721-727 bp	1.0	76.0
rbcL-accD_p3	GAGTCCTGAACTAGCTGCTG	ATCGAACCGCCATTTTTCCA	55.0	45.0	57.7	58.5	716-722 bp	1.0	75.0
rbcL-accD_p4	GAGTCCTGAACTAGCTGCTG	TCGAACCGCCATTTTTCCAT	55.0	45.0	57.7	58.5	715-721 bp	1.0	75.0
rbcL-accD_p5	GCTGCTGCTTGTGAAGTATG	CGAATCGAACCGCCATTTTT	50.0	45.0	57.5	57.7	706-712 bp	1.0	74.6