Hotspot

trnT-GGU-psbD

LSC · 33929-35377 · 1449 bp

Back to Phippsiomeles

Pi 0.00563
MarkerSeek score 60.00
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1449
variable_sites
8
pi
0.00563
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG GGCCAGACCAACCTACAAAA 55.0 50.0 58.1 58.0 1613-1701 bp 1.0 81.2
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA GGCCAGACCAACCTACAAAA 50.0 50.0 58.1 58.0 1616-1704 bp 1.0 81.0
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG TAGGCCAGACCAACCTACAA 55.0 50.0 58.1 58.0 1615-1703 bp 1.0 81.0
trnT-GGU-psbD_p4 GTGGTAGAGTAACGCCATGG ACCTACAAAAACGAAGCGGT 55.0 45.0 58.1 58.0 1603-1691 bp 1.0 80.9
trnT-GGU-psbD_p5 TCAGTGGTAGAGTAACGCCA TAGGCCAGACCAACCTACAA 50.0 50.0 58.1 58.0 1618-1706 bp 1.0 80.8