Hotspot

psbM-trnD-GUC

LSC · 31363-32603 · 1241 bp

Back to Phippsiomeles

Pi 0.00164
MarkerSeek score 61.60
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1241
variable_sites
2
pi
0.00164
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 TCACCTTGGCTGACTGTTTT TTCAATTGGTCAGAGCACCG 45.0 50.0 57.6 58.5 1386-1406 bp 1.0 74.2
psbM-trnD-GUC_p2 AATCACCTTGGCTGACTGTT TTCAATTGGTCAGAGCACCG 45.0 50.0 57.3 58.5 1388-1408 bp 1.0 72.4
psbM-trnD-GUC_p3 ATCACCTTGGCTGACTGTTT TTCAATTGGTCAGAGCACCG 45.0 50.0 57.3 58.5 1387-1407 bp 1.0 72.4
psbM-trnD-GUC_p4 TCACCTTGGCTGACTGTTTT GTTCAATTGGTCAGAGCACC 45.0 50.0 57.6 57.0 1387-1407 bp 1.0 70.1
psbM-trnD-GUC_p5 ATCACCTTGGCTGACTGTTTT TTCAATTGGTCAGAGCACCG 42.9 50.0 58.1 58.5 1387-1407 bp 1.0 69.3