Hotspot

atpF-atpH

LSC · 13494-13964 · 471 bp

Back to Phippsiomeles

Pi 0.00887

MarkerSeek score 65.20

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 471
variable_sites: 4
pi: 0.00887
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	CTGGTTGTCGCATTAGCTCT	50.0	50.0	57.9	58.0	568-585 bp	1.0	82.3
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	TGGACTGGTTGTCGCATTAG	50.0	50.0	57.9	57.9	572-589 bp	1.0	81.6
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	ACTGGTTGTCGCATTAGCTC	50.0	50.0	57.9	58.3	569-586 bp	1.0	80.2
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	GACTGGTTGTCGCATTAGCT	50.0	50.0	57.9	58.3	570-587 bp	1.0	80.2
atpF-atpH_p5	GATGGCCAATAACCCAAGGA	CTGGTTGTCGCATTAGCTCT	50.0	50.0	57.5	58.0	546-563 bp	1.0	79.7