Hotspot

ycf1

SSC · 125087-130531 · 5445 bp

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Pi 0.0188

MarkerSeek score 55.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5445
variable_sites: 197
pi: 0.0188
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGTGAACCTTATTTATCCAAAGT	AAAAAGGACTCAATTGTACAAT	30.4	27.3	53.9	52.2	173-176 bp	1.0	47.3
ycf1_p2	TTGTGAACCTTATTTATCCAAAGT	AAAAAGGACTCAATTGTACAAT	29.2	27.3	54.7	52.2	174-177 bp	1.0	47.3
ycf1_p3	TGTGAACCTTATTTATCCAAAG	AAAAAGGACTCAATTGTACAAT	31.8	27.3	52.1	52.2	173-176 bp	1.0	47.3
ycf1_p4	GTGAACCTTATTTATCCAAAGT	AAAAAGGACTCAATTGTACAAT	31.8	27.3	52.1	52.2	172-175 bp	1.0	47.3
ycf1_p5	ATTGTGAACCTTATTTATCCAAAGT	AAAAAGGACTCAATTGTACAAT	28.0	27.3	55.2	52.2	175-178 bp	1.0	47.2