Hotspot

ndhE-ndhG

SSC · 119190-119447 · 258 bp

Back to Philotheca

Pi 0.046

MarkerSeek score 71.70

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 258
variable_sites: 23
pi: 0.046
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	TCCTCTGTTCTTGGAGCAGA	TCGTGGTACGGGCTTATTTG	50.0	50.0	58.0	58.0	1125-1129 bp	1.0	83.4
ndhE-ndhG_p2	TCCTCTGTTCTTGGAGCAGA	GTCGTGGTACGGGCTTATTT	50.0	50.0	58.0	58.0	1126-1130 bp	1.0	83.4
ndhE-ndhG_p3	GCATCGCTTACAACCAACAC	TCGTGGTACGGGCTTATTTG	50.0	50.0	58.0	58.0	1147-1151 bp	1.0	83.2
ndhE-ndhG_p4	GCATCGCTTACAACCAACAC	GTCGTGGTACGGGCTTATTT	50.0	50.0	58.0	58.0	1148-1152 bp	1.0	83.2
ndhE-ndhG_p5	TCCTCTGTTCTTGGAGCAGA	AAATGCCTGGTCGGTTCAAT	50.0	45.0	58.0	58.1	1552-1556 bp	1.0	82.6