Hotspot

trnN-GUU-rpl32

SSC · 110205-114442 · 4238 bp

Back to Philotheca

Pi 0.0151

MarkerSeek score 53.00

Primer available yes

Label trnN-GUU-rpl32

Feature Metadata

label_name: trnN-GUU-rpl32
feature_type: igs
region: SSC
length_bp: 4238
variable_sites: 125
pi: 0.0151
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-rpl32_p1	TGTTGCGGTCGGAAAAAGTA	TTTATTGGGGAACGCTCTGG	45.0	50.0	58.0	57.9	480-486 bp	0.75	70.1
trnN-GUU-rpl32_p2	TTTTTGACAAGCACTTGCCG	TTTATTGGGGAACGCTCTGG	45.0	50.0	58.1	57.9	912-918 bp	0.75	69.8
trnN-GUU-rpl32_p3	GTTTTGTTGCGGTCGGAAAA	TTTATTGGGGAACGCTCTGG	45.0	50.0	58.1	57.9	484-490 bp	0.75	69.8
trnN-GUU-rpl32_p4	AAAAGACCACATACGGCGAA	TTTATTGGGGAACGCTCTGG	45.0	50.0	57.8	57.9	507-513 bp	0.75	69.4
trnN-GUU-rpl32_p5	ATAGGTCGCGTGAACCAAAA	TTTATTGGGGAACGCTCTGG	45.0	50.0	57.8	57.9	890-896 bp	0.75	69.0