Hotspot

rps3-rpl22

LSC · 84424-84662 · 239 bp

Back to Philotheca

Pi 0.0558

MarkerSeek score 75.50

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 239
variable_sites: 25
pi: 0.0558
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	CAAGTCGGAAACCAAGTGGA	GGTTTCAACGAAGCGAGTTT	50.0	45.0	58.0	57.2	307-312 bp	1.0	80.4
rps3-rpl22_p2	ACCAAGTCGGAAACCAAGTG	GGTTTCAACGAAGCGAGTTT	50.0	45.0	58.3	57.2	309-314 bp	1.0	78.1
rps3-rpl22_p3	CAAGTCGGAAACCAAGTGGA	GGGTTTCAACGAAGCGAGTT	50.0	50.0	58.0	59.1	308-313 bp	1.0	77.9
rps3-rpl22_p4	ACCAAGTCGGAAACCAAGTG	GGGTTTCAACGAAGCGAGTT	50.0	50.0	58.3	59.1	310-315 bp	1.0	75.6
rps3-rpl22_p5	CAAGTCGGAAACCAAGTGGA	GGGTTTCAACGAAGCGAGT	50.0	52.6	58.0	58.4	308-313 bp	1.0	75.3