Hotspot

petG-trnW-CCA

LSC · 67430-67580 · 151 bp

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Pi 0.053
MarkerSeek score 76.80
Primer available yes
Label petG-trnW-CCA

Feature Metadata

label_name
petG-trnW-CCA
feature_type
igs
region
LSC
length_bp
151
variable_sites
16
pi
0.053
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.96
markerseek_score
76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petG-trnW-CCA_p1 AGCTCCGTCATGCCTACTAT AGCGCTCTTAGTTCAGTTCG 50.0 50.0 58.0 58.0 628-635 bp 1.0 84.0
petG-trnW-CCA_p2 AGCTCCGTCATGCCTACTAT AGAACGTGGGTCTCCAAAAC 50.0 50.0 58.0 58.0 606-613 bp 1.0 84.0
petG-trnW-CCA_p3 GTTGATTCGGAATCGTGGGA AGCGCTCTTAGTTCAGTTCG 50.0 50.0 58.0 58.0 853-860 bp 1.0 83.9
petG-trnW-CCA_p4 AGCTCCGTCATGCCTACTAT TGCGCGGAATCCTTAATTCA 50.0 45.0 58.0 58.0 1146-1156 bp 1.0 83.8
petG-trnW-CCA_p5 AGCTCCGTCATGCCTACTAT GGACACTTGTATTTGTGCGC 50.0 50.0 58.0 58.0 1161-1171 bp 1.0 83.7