Hotspot

trnT-UGU-trnL-UAA

LSC · 47866-48865 · 1000 bp

Back to Philotheca

Pi 0.0355

MarkerSeek score 72.00

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 1000
variable_sites: 66
pi: 0.0355
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	TTGGAAAAGTGGGAAGAGGC	TACCAAAAAGGGGCAGTCAC	50.0	50.0	58.0	58.0	1397-1411 bp	0.75	72.4
trnT-UGU-trnL-UAA_p2	TTTTCCGGCGATACGAACAT	TACCAAAAAGGGGCAGTCAC	45.0	50.0	58.0	58.0	1897-1911 bp	0.75	72.0
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	TACCAAAAAGGGGCAGTCAC	55.0	50.0	57.9	58.0	1267-1281 bp	0.75	71.6
trnT-UGU-trnL-UAA_p4	TTGGAAAAGTGGGAAGAGGC	AACAGCTTCCGTTGAGTCTC	50.0	50.0	58.0	57.8	1364-1378 bp	0.75	71.2
trnT-UGU-trnL-UAA_p5	TTGGAAAAGTGGGAAGAGGC	GAACAGCTTCCGTTGAGTCT	50.0	50.0	58.0	57.8	1365-1379 bp	0.75	71.2