Hotspot

trnG-UCC-trnR-UCU

LSC · 9981-10167 · 187 bp

Back to Philotheca

Pi 0.0579

MarkerSeek score 77.90

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 187
variable_sites: 21
pi: 0.0579
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	ATCAATTGCGTCCAATAGGA	50.0	40.0	58.0	54.8	308-321 bp	1.0	59.8
trnG-UCC-trnR-UCU_p2	CCTAGCCTTCCAAGCTAACG	ATCAATTGCGTCCAATAGGA	55.0	40.0	58.1	54.8	311-324 bp	1.0	59.5
trnG-UCC-trnR-UCU_p3	AGCCTTCCAAGCTAACGATG	AATCAATTGCGTCCAATAGGA	50.0	38.1	58.0	55.6	309-322 bp	1.0	58.4
trnG-UCC-trnR-UCU_p4	CCTAGCCTTCCAAGCTAACG	AATCAATTGCGTCCAATAGGA	55.0	38.1	58.1	55.6	312-325 bp	1.0	58.2
trnG-UCC-trnR-UCU_p5	CCCTAGCCTTCCAAGCTAAC	ATCAATTGCGTCCAATAGGA	55.0	40.0	57.7	54.8	312-325 bp	1.0	57.3