Hotspot

psbI-trnS-GCU

LSC · 8033-8157 · 125 bp

Back to Philotheca

Pi 0.044

MarkerSeek score 75.80

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 125
variable_sites: 11
pi: 0.044
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	CTGTCTAATGATCCAGGGCG	TGAGTGGATTAAAGCGTCGG	55.0	50.0	57.8	58.0	243-246 bp	1.0	82.6
psbI-trnS-GCU_p2	CTGTCTAATGATCCAGGGCG	TAGGAGAGATGGCTGAGTGG	55.0	55.0	57.8	57.9	256-259 bp	1.0	81.8
psbI-trnS-GCU_p3	CTGTCTAATGATCCAGGGCG	GGCTGAGTGGATTAAAGCGT	55.0	50.0	57.8	58.3	246-249 bp	1.0	80.5
psbI-trnS-GCU_p4	CTGTCTAATGATCCAGGGCG	TGGCTGAGTGGATTAAAGCG	55.0	50.0	57.8	58.3	247-250 bp	1.0	80.5
psbI-trnS-GCU_p5	CTGTCTAATGATCCAGGGCG	GAGTGGATTAAAGCGTCGGA	55.0	50.0	57.8	57.7	242-245 bp	1.0	80.3