Hotspot

psbK-psbI

LSC · 7541-7921 · 381 bp

Back to Philotheca

Pi 0.0359

MarkerSeek score 66.50

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 381
variable_sites: 26
pi: 0.0359
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	ACAAAGAGTTTGAGAGTAAGCA	50.0	36.4	57.9	55.6	433-435 bp	1.0	49.8
psbK-psbI_p2	TGGCAAGCTGCTGTAAGTTT	ACAAAGAGTTTGAGAGTAAGCA	45.0	36.4	58.3	55.6	430-432 bp	1.0	47.8
psbK-psbI_p3	TTGGCAAGCTGCTGTAAGTT	ACAAAGAGTTTGAGAGTAAGCA	45.0	36.4	58.3	55.6	431-433 bp	1.0	47.8
psbK-psbI_p4	TTTGGCAAGCTGCTGTAAGT	ACAAAGAGTTTGAGAGTAAGCA	45.0	36.4	58.3	55.6	432-434 bp	1.0	47.8
psbK-psbI_p5	GTTTGGCAAGCTGCTGTAAG	ACAAAGAGTTTGAGAGTAAGCAT	50.0	34.8	57.9	56.1	433-435 bp	1.0	45.9