Hotspot

trnK-UUU-rps16

LSC · 4391-4976 · 586 bp

Back to Philotheca

Pi 0.0348

MarkerSeek score 71.90

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 586
variable_sites: 37
pi: 0.0348
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGCCGTACTTAAAAGCCGAG	ATTTCAAAGAAAGCGGGGGT	50.0	45.0	58.0	58.0	668-691 bp	1.0	88.6
trnK-UUU-rps16_p2	AATTGGAACCCTAGGGCTCT	ATTTCAAAGAAAGCGGGGGT	50.0	45.0	58.0	58.0	911-939 bp	1.0	87.6
trnK-UUU-rps16_p3	AGCCGTACTTAAAAGCCGAG	CCCAATGAGCCGTTTATCGA	50.0	50.0	58.0	58.1	841-864 bp	1.0	86.9
trnK-UUU-rps16_p4	AGCCGTACTTAAAAGCCGAG	TCCCAATGAGCCGTTTATCG	50.0	50.0	58.0	58.1	842-865 bp	1.0	86.9
trnK-UUU-rps16_p5	GGTAAGGAAGTTAGTGGGCG	ATTTCAAAGAAAGCGGGGGT	55.0	45.0	58.0	58.0	1352-1380 bp	1.0	85.9