Hotspot

psbA-trnK-UUU

LSC · 1506-1772 · 267 bp

Back to Philotheca

Pi 0.0362

MarkerSeek score 73.50

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 267
variable_sites: 18
pi: 0.0362
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	ATGTAAAGGCGGTTTTCGGT	ACTCCATCCGACTAGTTCCG	45.0	55.0	58.1	58.6	393-404 bp	1.0	80.0
psbA-trnK-UUU_p2	ACCATCCAATGTAAAGGCGG	ACTCCATCCGACTAGTTCCG	50.0	55.0	58.2	58.6	401-412 bp	1.0	78.8
psbA-trnK-UUU_p3	CCATCCAATGTAAAGGCGGT	ACTCCATCCGACTAGTTCCG	50.0	55.0	58.2	58.6	400-411 bp	1.0	78.8
psbA-trnK-UUU_p4	ATGTAAAGGCGGTTTTCGGT	ACTAGTTCCGGGTTCGAGTC	45.0	55.0	58.1	58.8	383-394 bp	1.0	78.4
psbA-trnK-UUU_p5	ATGTAAAGGCGGTTTTCGGT	GACTAGTTCCGGGTTCGAGT	45.0	55.0	58.1	58.8	384-395 bp	1.0	78.3