Hotspot

ndhI

SSC · 130279-130821 · 543 bp

Back to Philodendron

Pi 0.488

MarkerSeek score 80.40

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 543
variable_sites: 233
pi: 0.488
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	CTGGAATAGCGACGGAAACA	TGTAGTGGATTAGTGTAGATATATT	50.0	28.0	58.0	52.6	1974 bp	0.5	20.0
ndhI_p2	TGGAATAGCGACGGAAACAG	TGTAGTGGATTAGTGTAGATATATT	50.0	28.0	58.0	52.6	1973 bp	0.5	20.0
ndhI_p3	GGAATAGCGACGGAAACAGT	TGTAGTGGATTAGTGTAGATATATT	50.0	28.0	58.0	52.6	1972 bp	0.5	20.0
ndhI_p4	TTTTGGGGTGGAATCTGTGG	TGTAGTGGATTAGTGTAGATATATT	50.0	28.0	58.0	52.6	1877 bp	0.5	20.0
ndhI_p5	TAGCGACGGAAACAGTGATG	TGTAGTGGATTAGTGTAGATATATT	50.0	28.0	58.0	52.6	1968 bp	0.5	20.0