Hotspot

ndhF

SSC · 138946-141174 · 2229 bp

Back to Philodendron

Pi 0.46

MarkerSeek score 78.90

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2229
variable_sites: 856
pi: 0.46
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AGAGTTGGTGAATCGGAAACA	TCTCTTATCTTACCGTCATACT	42.9	36.4	57.5	53.3	2549 bp	0.5	20.0
ndhF_p2	AGAGTTGGTGAATCGGAAACA	TCTCTTATCTTACCGTCATACTT	42.9	34.8	57.5	54.1	2549 bp	0.5	20.0
ndhF_p3	AGAGTTGGTGAATCGGAAACA	TTCTCTTATCTTACCGTCATACT	42.9	34.8	57.5	54.1	2550 bp	0.5	20.0
ndhF_p4	AGAGTTGGTGAATCGGAAACA	TTCTCTTATCTTACCGTCATACTT	42.9	33.3	57.5	54.9	2550 bp	0.5	20.0
ndhF_p5	AGAGTTGGTGAATCGGAAACA	TTTCTCTTATCTTACCGTCATACT	42.9	33.3	57.5	54.9	2551 bp	0.5	20.0