Hotspot

ndhD

SSC · 133969-135471 · 1503 bp

Back to Philodendron

Pi 0.48

MarkerSeek score 77.70

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 534
pi: 0.48
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.74
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GCATGAGACAACTCGCAGTA	GGGCTCCTATTGGAATTGGG	50.0	55.0	58.0	58.0	2104 bp	0.5	60.0
ndhD_p2	GCATGAGACAACTCGCAGTA	GGGGCTCCTATTGGAATTGG	50.0	55.0	58.0	58.0	2105 bp	0.5	60.0
ndhD_p3	TATGGCATGAGACAACTCGC	GGGCTCCTATTGGAATTGGG	50.0	55.0	58.1	58.0	2108 bp	0.5	59.5
ndhD_p4	TATGGCATGAGACAACTCGC	GGGGCTCCTATTGGAATTGG	50.0	55.0	58.1	58.0	2109 bp	0.5	59.5
ndhD_p5	GCATGAGACAACTCGCAGTA	CCCTAAAGAAACTTGGGCGT	50.0	50.0	58.0	58.1	2083 bp	0.5	59.2