Hotspot

ndhA

SSC · 127988-130179 · 2192 bp

Back to Philodendron

Pi 0.472

MarkerSeek score 80.80

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2192
variable_sites: 933
pi: 0.472
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	CAATGTTACGAGCTTCCGGA	CAATATACCTTGCAGCCCGT	50.0	50.0	58.0	58.0	2877 bp	0.5	59.7
ndhA_p2	CAATGTTACGAGCTTCCGGA	CCCGTACTGTTTGTTGACCA	50.0	50.0	58.0	58.0	2862 bp	0.5	59.6
ndhA_p3	TCGGTGAAATGACGGAATCC	CAATATACCTTGCAGCCCGT	50.0	50.0	58.0	58.0	2748 bp	0.5	59.6
ndhA_p4	TCGGTGAAATGACGGAATCC	CCCGTACTGTTTGTTGACCA	50.0	50.0	58.0	58.0	2733 bp	0.5	59.5
ndhA_p5	GGCTTATCGGGACCAATGTT	CAATATACCTTGCAGCCCGT	50.0	50.0	57.9	58.0	2890 bp	0.5	59.3