Hotspot

rrn5-rrn4.5

SSC · 144561-144784 · 224 bp

Back to Philodendron

Pi 0.0491
MarkerSeek score 80.70
Primer available yes
Label rrn5-rrn4.5

Feature Metadata

label_name
rrn5-rrn4.5
feature_type
igs
region
SSC
length_bp
224
variable_sites
11
pi
0.0491
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn5-rrn4.5_p1 AGTATCGTCACCGCAGTAGA CAGCTGAGGCATCCTAACAG 50.0 55.0 58.0 58.0 341-349 bp 1.0 81.9
rrn5-rrn4.5_p2 TATCGTCACCGCAGTAGAGT CAGCTGAGGCATCCTAACAG 50.0 55.0 58.0 58.0 339-347 bp 1.0 81.9
rrn5-rrn4.5_p3 TATCGTCACCGCAGTAGAGT GCTGAGGCATCCTAACAGAC 50.0 55.0 58.0 58.1 337-345 bp 1.0 81.9
rrn5-rrn4.5_p4 AGTATCGTCACCGCAGTAGA GTCAAGTGGAAGTGCAGTGA 50.0 50.0 58.0 58.0 367-375 bp 1.0 81.7
rrn5-rrn4.5_p5 TATCGTCACCGCAGTAGAGT GTCAAGTGGAAGTGCAGTGA 50.0 50.0 58.0 58.0 365-373 bp 1.0 81.7