Hotspot

ndhF-trnN

SSC · 141175-143460 · 2286 bp

Back to Philodendron

Pi 0.263

MarkerSeek score 80.40

Primer available yes

Label ndhF-trnN

Feature Metadata

label_name: ndhF-trnN
feature_type: igs
region: SSC
length_bp: 2286
variable_sites: 536
pi: 0.263
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-trnN_p1	ACAAACAGCGTTGGGGTTAT	CCAGGTATTGACTGACGGTG	45.0	55.0	58.0	58.0	2662 bp	0.5	59.8
ndhF-trnN_p2	ATTACAAACAGCGTTGGGGT	CCAGGTATTGACTGACGGTG	45.0	55.0	58.0	58.0	2665 bp	0.5	59.8
ndhF-trnN_p3	ACAAACAGCGTTGGGGTTAT	CGGGAGAATTGATGACTGCA	45.0	50.0	58.0	58.0	2778 bp	0.5	59.7
ndhF-trnN_p4	ATTACAAACAGCGTTGGGGT	CGGGAGAATTGATGACTGCA	45.0	50.0	58.0	58.0	2781 bp	0.5	59.7
ndhF-trnN_p5	TTTCTTTGCGGGTGAAGGAA	CCAGGTATTGACTGACGGTG	45.0	55.0	57.9	58.0	2639 bp	0.5	59.4