Hotspot

ndhI-ndhG

SSC · 130822-131725 · 904 bp

Back to Philodendron

Pi 0.423

MarkerSeek score 81.60

Primer available yes

Label ndhI-ndhG

Feature Metadata

label_name: ndhI-ndhG
feature_type: igs
region: SSC
length_bp: 904
variable_sites: 333
pi: 0.423
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhG_p1	ACGGGCTGCAAGGTATATTG	ACAAGAACCGATCCCAGAGA	50.0	50.0	58.0	58.1	1531 bp	0.5	59.2
ndhI-ndhG_p2	TGGTCAACAAACAGTACGGG	ACAAGAACCGATCCCAGAGA	50.0	50.0	58.0	58.1	1546 bp	0.5	59.2
ndhI-ndhG_p3	ACGGGCTGCAAGGTATATTG	CCCTCATGAACATCACAGCA	50.0	50.0	58.0	57.9	1647 bp	0.5	58.8
ndhI-ndhG_p4	TGGTCAACAAACAGTACGGG	CCCTCATGAACATCACAGCA	50.0	50.0	58.0	57.9	1662 bp	0.5	58.7
ndhI-ndhG_p5	ACGGGCTGCAAGGTATATTG	ACAAACTAGCGAAGTGACCC	50.0	50.0	58.0	57.8	1712 bp	0.5	58.4