Hotspot

petD-rpoA

LSC · 87356-87546 · 191 bp

Back to Philodendron

Pi 0.0802

MarkerSeek score 82.90

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 191
variable_sites: 15
pi: 0.0802
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	TTTTTGATCGGTACCGCAGT	AGACATTCTACAGAAACATTTCACA	45.0	32.0	57.8	57.0	333-336 bp	1.0	44.5
petD-rpoA_p2	CGTCGTCCAGTAGCTACAAC	AGACATTCTACAGAAACATTTCACA	55.0	32.0	58.1	57.0	357-360 bp	1.0	44.2
petD-rpoA_p3	TCGTCGTCCAGTAGCTACAA	AGACATTCTACAGAAACATTTCACA	50.0	32.0	58.2	57.0	358-361 bp	1.0	44.2
petD-rpoA_p4	TTCGTCGTCCAGTAGCTACA	AGACATTCTACAGAAACATTTCACA	50.0	32.0	58.2	57.0	359-362 bp	1.0	44.2
petD-rpoA_p5	CATTTCGTCGTCCAGTAGCT	AGACATTCTACAGAAACATTTCACA	50.0	32.0	57.7	57.0	362-365 bp	1.0	44.2