Hotspot

psbC-trnS-UGA

LSC · 41546-41668 · 123 bp

Back to Philodendron

Pi 0.0984

MarkerSeek score 88.00

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 123
variable_sites: 12
pi: 0.0984
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 88.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CCTGTTCTTTCCATGACCCC	GGGTTCGAATCCCTCTCTCT	55.0	55.0	58.2	58.2	175-176 bp	1.0	82.9
psbC-trnS-UGA_p2	CCTGTTCTTTCCATGACCCC	GGTTGATAGCTCCGGTCTTG	55.0	55.0	58.2	58.1	229-230 bp	1.0	82.8
psbC-trnS-UGA_p3	TTCCATGACCCCTCTTAGCT	GGTTGATAGCTCCGGTCTTG	50.0	55.0	57.7	58.1	221-222 bp	1.0	82.1
psbC-trnS-UGA_p4	CCTGTTCTTTCCATGACCCC	GATGGCCGAGTGGTTGATAG	55.0	55.0	58.2	58.1	240-241 bp	1.0	82.0
psbC-trnS-UGA_p5	TTGTGGGTCATTTGTGGCAT	GGTTGATAGCTCCGGTCTTG	45.0	55.0	58.3	58.1	309-310 bp	1.0	80.6