Hotspot

trnH-GUG-psbA

LSC · 78-791 · 714 bp

Back to Philodendron

Pi 0.0691
MarkerSeek score 79.90
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
714
variable_sites
46
pi
0.0691
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG TAGTTGACAGTCAGGGTCGT 55.0 50.0 58.1 58.0 881-930 bp 1.0 87.1
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG CCTCTAGACCTAGCTGCTGT 55.0 55.0 58.1 57.9 780-829 bp 1.0 87.1
trnH-GUG-psbA_p3 CAATCCACTGCCTTGATCCA TAGTTGACAGTCAGGGTCGT 50.0 50.0 57.9 58.0 895-944 bp 1.0 87.0
trnH-GUG-psbA_p4 AATCCACTGCCTTGATCCAC TAGTTGACAGTCAGGGTCGT 50.0 50.0 57.9 58.0 894-943 bp 1.0 86.9
trnH-GUG-psbA_p5 ACAATCCACTGCCTTGATCC TAGTTGACAGTCAGGGTCGT 50.0 50.0 57.9 58.0 896-945 bp 1.0 86.9