Hotspot

rbcL

LSC · 57208-58635 · 1428 bp

Back to Phillyrea

Pi 0.00467

MarkerSeek score 49.20

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1428
variable_sites: 10
pi: 0.00467
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	AACGCCTAATTCGTGTCGAG	AAGATTGGGCCGAGTTTTCA	50.0	45.0	58.4	57.7	1556 bp	1.0	75.2
rbcL_p2	AACGCCTAATTCGTGTCGAG	AGATTGGGCCGAGTTTTCAA	50.0	45.0	58.4	57.7	1555 bp	1.0	75.2
rbcL_p3	CGCCTAATTCGTGTCGAGTA	AAGATTGGGCCGAGTTTTCA	50.0	45.0	57.5	57.7	1554 bp	1.0	74.5
rbcL_p4	CGCCTAATTCGTGTCGAGTA	AGATTGGGCCGAGTTTTCAA	50.0	45.0	57.5	57.7	1553 bp	1.0	74.5
rbcL_p5	TAACGCCTAATTCGTGTCGA	AGATTGGGCCGAGTTTTCAA	45.0	45.0	57.1	57.7	1556 bp	1.0	70.9