Hotspot

rps12-trnV(GAC)

IRb · 100533-102128 · 1596 bp

Back to Phillyrea

Pi 0.00125
MarkerSeek score 59.90
Primer available yes
Label rps12-trnV(GAC)

Feature Metadata

label_name
rps12-trnV(GAC)
feature_type
igs
region
IRb
length_bp
1596
variable_sites
3
pi
0.00125
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-trnV(GAC)_p1 TGGCAACTTTACGTAAGGCA GATGACTTCCACCACGTCAA 45.0 50.0 57.7 57.8 1684-1692 bp 1.0 77.1
rps12-trnV(GAC)_p2 TGGCAACTTTACGTAAGGCA AACTGATGACTTCCACCACG 45.0 50.0 57.7 57.8 1688-1696 bp 1.0 77.1
rps12-trnV(GAC)_p3 TGGCAACTTTACGTAAGGCA ATGACTTCCACCACGTCAAG 45.0 50.0 57.7 57.8 1683-1691 bp 1.0 77.1
rps12-trnV(GAC)_p4 TGGCAACTTTACGTAAGGCA CTGATGACTTCCACCACGTC 45.0 55.0 57.7 58.3 1686-1694 bp 1.0 76.2
rps12-trnV(GAC)_p5 TGGCAACTTTACGTAAGGCA GTCAAGGTGACACTCTACCG 45.0 55.0 57.7 57.7 1669-1677 bp 1.0 75.7