Hotspot

atpB-rbcL

LSC · 56402-57207 · 806 bp

Back to Phillyrea

Pi 0.00331

MarkerSeek score 62.80

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 806
variable_sites: 4
pi: 0.00331
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	ATACGGCCCAGGTTTTGTTT	AACACCCGCTTTGAATCCAA	45.0	45.0	58.0	58.3	911-925 bp	1.0	79.1
atpB-rbcL_p2	GGAAACCCCAGAACCAGAAG	AACACCCGCTTTGAATCCAA	55.0	45.0	58.1	58.3	882-896 bp	1.0	78.5
atpB-rbcL_p3	GGCGGAAAGGCTACATCTAG	AACACCCGCTTTGAATCCAA	55.0	45.0	57.8	58.3	953-967 bp	1.0	77.8
atpB-rbcL_p4	TACGGCCCAGGTTTTGTTTT	AACACCCGCTTTGAATCCAA	45.0	45.0	58.2	58.3	910-924 bp	1.0	77.4
atpB-rbcL_p5	GGACCGATGATTTGGACGAT	AACACCCGCTTTGAATCCAA	50.0	45.0	57.8	58.3	929-943 bp	1.0	77.2