Hotspot

psaA-ycf3

LSC · 43542-44285 · 744 bp

Back to Phillyrea

Pi 0.00358

MarkerSeek score 61.30

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 744
variable_sites: 4
pi: 0.00358
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	GGAAACAGGCTATAGCGCTT	50.0	50.0	57.9	58.0	902-905 bp	1.0	79.2
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	ATTGGTTGAAGATCACGGGG	50.0	50.0	57.9	57.9	854-857 bp	1.0	78.6
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	TGGTTTGATCAAGCAGCTGA	50.0	45.0	57.9	57.7	927-930 bp	1.0	77.4
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	AGGCTATAGCGCTTACTCCT	50.0	50.0	57.9	57.7	896-899 bp	1.0	77.2
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	TTGGAAACAGGCTATAGCGC	50.0	50.0	57.9	58.3	904-907 bp	1.0	76.9