Hotspot

rps16-trnQ(UUG)

LSC · 6441-8101 · 1661 bp

Back to Phillyrea

Pi 0.00403
MarkerSeek score 53.30
Primer available yes
Label rps16-trnQ(UUG)

Feature Metadata

label_name
rps16-trnQ(UUG)
feature_type
igs
region
LSC
length_bp
1661
variable_sites
10
pi
0.00403
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ(UUG)_p1 CGGATCTGTCCTTCAAGTCG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.1 58.1 1743-1748 bp 1.0 79.4
rps16-trnQ(UUG)_p2 ATCTGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 1740-1745 bp 1.0 79.4
rps16-trnQ(UUG)_p3 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1729-1734 bp 1.0 79.0
rps16-trnQ(UUG)_p4 CCACAACGGATCTGTCCTTC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.3 58.1 1749-1754 bp 1.0 77.9
rps16-trnQ(UUG)_p5 GGATCTGTCCTTCAAGTCGC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 1742-1747 bp 1.0 77.3