Hotspot

ycf1

SSC · 126580-132258 · 5679 bp

Back to Philadelphus

Pi 0.00264

MarkerSeek score 43.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5679
variable_sites: 15
pi: 0.00264
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGCTTCCATTCCCCAAACTG	TCCTGAGTGGTCTGAGGATT	50.0	50.0	58.1	57.4	259 bp	1.0	75.3
ycf1_p2	AGGTTAGCTTCCATTCCCCA	TCCTGAGTGGTCTGAGGATT	50.0	50.0	58.3	57.4	264 bp	1.0	73.3
ycf1_p3	GCTTCCATTCCCCAAACTGT	TCCTGAGTGGTCTGAGGATT	50.0	50.0	58.4	57.4	258 bp	1.0	72.9
ycf1_p4	AGCTTCCATTCCCCAAACTG	TGCAATTTCCTGAGTGGTCT	50.0	45.0	58.1	57.0	266 bp	1.0	72.7
ycf1_p5	GGTTAGCTTCCATTCCCCAA	TCCTGAGTGGTCTGAGGATT	50.0	50.0	57.5	57.4	263 bp	1.0	71.7