Hotspot

trnQ-UUG

LSC · 7832-7903 · 72 bp

Back to Philadelphus

Pi 0.0139
MarkerSeek score 60.50
Primer available yes
Label trnQ-UUG

Feature Metadata

label_name
trnQ-UUG
feature_type
tRNA
region
LSC
length_bp
72
variable_sites
1
pi
0.0139
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG_p1 TCCAAAACACAATCTTGATTCTGA GGTTGGAATTGACGAAAAACCA 33.3 40.9 57.0 58.0 175 bp 1.0 40.5
trnQ-UUG_p2 TCCAAAACACAATCTTGATTCTGA TGGGTTGGAATTGACGAAAAAC 33.3 40.9 57.0 58.0 177 bp 1.0 40.5
trnQ-UUG_p3 TCCAAAACACAATCTTGATTCTGA TGGGTTGGAATTGACGAAAA 33.3 40.0 57.0 55.8 177 bp 1.0 40.5
trnQ-UUG_p4 TCCAAAACACAATCTTGATTCTGA TTGGGTTGGAATTGACGAAA 33.3 40.0 57.0 55.8 178 bp 1.0 40.4
trnQ-UUG_p5 TCCAAAACACAATCTTGATTCTGA TTTGGGTTGGAATTGACGAA 33.3 40.0 57.0 55.8 179 bp 1.0 40.4