Hotspot

psbA

LSC · 421-1482 · 1062 bp

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Pi 0

MarkerSeek score 35.10

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	GCAATACCGCCCTCTTGATA	ATTTGTGTGCTTGGGAGTCC	50.0	50.0	57.5	58.4	1199 bp	1.0	72.6
psbA_p2	AAAAAGGAGCAATACCGCCC	ATTTGTGTGCTTGGGAGTCC	50.0	50.0	58.8	58.4	1207 bp	1.0	70.4
psbA_p3	ACCGCCCTCTTGATAAAACA	ATTTGTGTGCTTGGGAGTCC	45.0	50.0	57.1	58.4	1194 bp	1.0	70.0
psbA_p4	AAAAGGAGCAATACCGCCC	ATTTGTGTGCTTGGGAGTCC	52.6	50.0	58.1	58.4	1206 bp	1.0	68.0
psbA_p5	ACCGCCCTCTTGATAAAACAA	ATTTGTGTGCTTGGGAGTCC	42.9	50.0	57.9	58.4	1194 bp	1.0	67.8