Hotspot

ndhD

SSC · 118296-119798 · 1503 bp

Back to Philadelphus

Pi 0.000665

MarkerSeek score 48.90

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 1
pi: 0.000665
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TTTCAGTGTACAACGAACGA	CGAGTACGGGTTTTTCTGGT	40.0	50.0	55.3	57.8	1604 bp	1.0	57.3
ndhD_p2	TTTCAGTGTACAACGAACGA	CCCGTGCCCGAAAATAAGAT	40.0	50.0	55.3	58.3	1640 bp	1.0	56.0
ndhD_p3	TTTTCAGTGTACAACGAACGA	CGAGTACGGGTTTTTCTGGT	38.1	50.0	56.1	57.8	1605 bp	1.0	55.6
ndhD_p4	TTTCAGTGTACAACGAACGA	AAAACCCGTGCCCGAAAATA	40.0	45.0	55.3	58.4	1644 bp	1.0	55.5
ndhD_p5	TTTCAGTGTACAACGAACGA	AAACCCGTGCCCGAAAATAA	40.0	45.0	55.3	58.4	1643 bp	1.0	55.5