Hotspot

ccsA-ndhD

SSC · 118051-118295 · 245 bp

Back to Philadelphus

Pi 0.0163

MarkerSeek score 61.90

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 245
variable_sites: 4
pi: 0.0163
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	ATTCTGCGATTGTGGCTTCT	ACCCGGATTTCGTTCTTTCA	45.0	45.0	57.9	57.4	432-442 bp	1.0	75.3
ccsA-ndhD_p2	ATTCTGCGATTGTGGCTTCT	ACCCGGATTTCGTTCTTTCAT	45.0	42.9	57.9	57.9	432-442 bp	1.0	71.2
ccsA-ndhD_p3	CAAATTCTGCGATTGTGGCT	ACCCGGATTTCGTTCTTTCA	45.0	45.0	57.4	57.4	435-445 bp	1.0	71.2
ccsA-ndhD_p4	TTCTGCGATTGTGGCTTCTA	ACCCGGATTTCGTTCTTTCA	45.0	45.0	57.2	57.4	431-441 bp	1.0	70.2
ccsA-ndhD_p5	ATTCTGCGATTGTGGCTTCT	TCAGTTGACAAGGTCGAAGT	45.0	45.0	57.9	56.7	409-419 bp	1.0	69.6