Hotspot

trnT-UGU-trnL-UAA

LSC · 48046-48710 · 665 bp

Back to Philadelphus

Pi 0.00451

MarkerSeek score 61.30

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 665
variable_sites: 3
pi: 0.00451
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	55.0	50.0	57.9	58.0	715-717 bp	1.0	79.2
trnT-UGU-trnL-UAA_p2	GAGTCGAACCGATGACCATC	ACCAATTTCGCCATATCCCC	55.0	50.0	58.2	58.0	746-748 bp	1.0	79.0
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	55.0	50.0	57.9	58.1	723-725 bp	1.0	78.7
trnT-UGU-trnL-UAA_p4	GAGTCGAACCGATGACCATC	TAGCGTCTACCAATTTCGCC	55.0	50.0	58.2	58.1	754-756 bp	1.0	78.5
trnT-UGU-trnL-UAA_p5	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	55.0	50.0	57.9	58.2	729-731 bp	1.0	77.3