Hotspot

matK-trnK-UUU

LSC · 3508-4209 · 702 bp

Back to Philadelphus

Pi 0.0057

MarkerSeek score 62.00

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 702
variable_sites: 4
pi: 0.0057
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	CCATGATCATGAGCAAGTGC	AACGGTAGAGTACTCGGCTT	50.0	50.0	57.2	58.2	833 bp	1.0	72.8
matK-trnK-UUU_p2	CCATGATCATGAGCAAGTGC	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.2	58.2	832 bp	1.0	72.8
matK-trnK-UUU_p3	CCATGATCATGAGCAAGTGC	GGGTTGCTAACTCAACGGTA	50.0	50.0	57.2	57.5	846 bp	1.0	70.5
matK-trnK-UUU_p4	AGGAAGTCGTGTTGCTGATA	AACGGTAGAGTACTCGGCTT	45.0	50.0	56.6	58.2	782 bp	1.0	67.7
matK-trnK-UUU_p5	AGGAAGTCGTGTTGCTGATA	ACGGTAGAGTACTCGGCTTT	45.0	50.0	56.6	58.2	781 bp	1.0	67.7