Hotspot

psbA-trnK-UUU

LSC · 1483-1697 · 215 bp

Back to Philadelphus

Pi 0.014

MarkerSeek score 60.50

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 215
variable_sites: 3
pi: 0.014
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	CAATTACAGAAGCGACCCCA	GAAAGCCGTGTGCAATGAAA	50.0	45.0	57.9	57.9	390 bp	1.0	78.7
psbA-trnK-UUU_p2	CAATTACAGAAGCGACCCCA	CGGCTTGGGGAGAGATTTTT	50.0	50.0	57.9	58.2	360 bp	1.0	78.5
psbA-trnK-UUU_p3	CAATTACAGAAGCGACCCCA	CTAGTTCCGGGTTCGAATCC	50.0	55.0	57.9	57.8	305 bp	1.0	78.2
psbA-trnK-UUU_p4	ACGGTTTTCAGTGCTGGTTA	GAAAGCCGTGTGCAATGAAA	45.0	45.0	57.7	57.9	412 bp	1.0	76.8
psbA-trnK-UUU_p5	ACGGTTTTCAGTGCTGGTTA	CGGCTTGGGGAGAGATTTTT	45.0	50.0	57.7	58.2	382 bp	1.0	76.7