Hotspot

rps16

LSC · 4943-6108 · 1166 bp

Back to Phellodendron

Pi 0.00172

MarkerSeek score 59.70

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1166
variable_sites: 2
pi: 0.00172
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TATAAGACTCCTCCCCACCC	AGACTGTGTATAGACTGAAGCA	55.0	40.9	57.2	56.3	1289 bp	1.0	44.4
rps16_p2	CTCCTCCCCACCCTCTTTTT	AGACTGTGTATAGACTGAAGCA	55.0	40.9	58.9	56.3	1282 bp	1.0	43.3
rps16_p3	ATAAGACTCCTCCCCACCCT	AGACTGTGTATAGACTGAAGCA	55.0	40.9	59.0	56.3	1288 bp	1.0	42.9
rps16_p4	TATAAGACTCCTCCCCACCC	AAGACTGTGTATAGACTGAAGCA	55.0	39.1	57.2	57.0	1290 bp	1.0	41.9
rps16_p5	TATAAGACTCCTCCCCACCC	AGACTGTGTATAGACTGAAGCAA	55.0	39.1	57.2	57.0	1289 bp	1.0	41.9