Hotspot

rpl22

IRa · 158221-158442 · 222 bp

Back to Phellodendron

Pi 0

MarkerSeek score 56.70

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: IRa
length_bp: 222
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	TCAATTTCCGTGGACATGCA	TGCTGCTGCAGAATAAACCA	45.0	45.0	58.1	58.1	284 bp	1.0	78.5
rpl22_p2	GGAGAATTTGCGCCCACTAT	TGCTGCTGCAGAATAAACCA	50.0	45.0	58.3	58.1	303 bp	1.0	76.7
rpl22_p3	CGCCCACTATCAATTTCCGT	TGCTGCTGCAGAATAAACCA	50.0	45.0	58.3	58.1	293 bp	1.0	76.6
rpl22_p4	AAATTGGGAGAATTTGCGCC	TGCTGCTGCAGAATAAACCA	45.0	45.0	57.6	58.1	309 bp	1.0	76.2
rpl22_p5	AGAATTTGCGCCCACTATCA	TGCTGCTGCAGAATAAACCA	45.0	45.0	57.6	58.1	301 bp	1.0	75.9