Hotspot

matK

LSC · 2144-3664 · 1521 bp

Back to Phellodendron

Pi 0

MarkerSeek score 40.00

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1521
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	AATTACATGGTCTCGTAACC	CGCCCACTAAATCCCTTACC	40.0	55.0	52.7	58.0	1593 bp	1.0	40.0
matK_p2	AATTACATGGTCTCGTAACC	GTATCGTTTGATCGCCCACT	40.0	50.0	52.7	58.1	1605 bp	1.0	40.0
matK_p3	AAATTACATGGTCTCGTAACC	CGCCCACTAAATCCCTTACC	38.1	55.0	53.6	58.0	1594 bp	1.0	40.0
matK_p4	AAATTACATGGTCTCGTAACC	GTATCGTTTGATCGCCCACT	38.1	50.0	53.6	58.1	1606 bp	1.0	40.0
matK_p5	AATTACATGGTCTCGTAACC	TGTTTTCACCGTATCGCACT	40.0	45.0	52.7	57.8	1627 bp	1.0	40.0